Epizootology and experimental infection of Yokose virus in bats

Issue Date

10-2008

Abstract

To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV. © 2008 Elsevier Ltd. All rights reserved.

Source or Periodical Title

Comparative Immunology, Microbiology and Infectious Diseases

ISSN

0147-9571

Volume

33

Issue

1

Page

25-36

Document Type

Article

Language

English

Subject

Anti-bat IgG, Bat, Chiroptera, ELISA, Experimental infection, Yokose virus

Identifier

doi:10.1016/j.cimid.2008.07.008.

Digital Copy

yes

Link to Full Text

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