Genetic characteristics of Fusarium verticillioides from corn in the Philippines

Issue Date

12-2009

Abstract

Fusarium verticillioides (teleomorph: Gibberella moniliformis = G. fujikuroi mating population A) is one of the most important fungal pathogens of corn worldwide. The pathogen produces fumonisins, mycotoxins that are potentially harmful to humans and animals. Thirty-five Fusarium isolates from Laguna and Isabela, Philippines were identified morphologically and molecularly as F. verticillioides and characterized by PCR for mating type (MAT). Twenty-six isolates were MAT1-2, while nine isolates were MAT1-1. The isolates from Isabela were tested for aggressiveness, rated according to a disease index (%) on 'Super Sweet' corn IPB variety 1 under field conditions across two trials using the toothpick inoculation method. Other aggressiveness traits such as inhibition of seedling emergence, decrease of seedling height, fresh and dry mass were also determined in two greenhouse trials. All isolates were pathogenic to corn seedlings and mature plants compared to the noninoculated control. Significant genotypic variation was observed (P = 0.01) in trial, isolate, and isolate × trial interaction for all traits across two greenhouse trials and that aggressiveness was highly influenced by the trial conditions. Similarly, significant genotypic variation was observed in trial, replication within trial and isolate × trial interaction. Heritability was high for the five traits in the greenhouse (h2 = 0.80-0.90) but moderate for disease index in the field (h2 = 0.49). In an analysis of fumonisin production in corn culture by high performance liquid chromatography, 30 of 35 strains produced a detectable level of fumonisins, varying from 0.44 to 742 μg FB1/g corn, 0.51-222 μg FB2/g and 0.12-37 μg FB3/g. Isabela isolates produced more fumonisins than the Laguna isolates did. In vitro fumonisin production had little correlation with the field disease index (r = 0.32) or with greenhouse seedling germination (0.25). © The Phytopathological Society of Japan and Springer 2009.

Source or Periodical Title

Journal of General Plant Pathology

ISSN

1345-2630

Volume

75

Issue

6

Page

405-412

Document Type

Article

Physical Description

tables, graphs

Language

English

Subject

Aggressiveness, Corn, F. verticillioides, Fumonisins, Mating type

Identifier

doi:10.1007/s10327-009-0199-4.

Digital Copy

yes

Link to Full Text

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