Selection of high ethanol-producing saccharomyces cerevisiae strains, their fermentation properties, and genetic differentiation based on rep-PCR and mt rDNA-PCR

Issue Date

6-2009

Abstract

Ten out of 37 Saccharomyces isolates statistically gave higher ethanol yields after 24 hours using 20% (total sugars) unsterile molasses medium. Saccharomyces cerevisiae 2025 and 2023 produced the highest ethanol concentrations of 8.7 % and 7.5% (v/v), respectively. In sterile molasses medium, ethanol production decreased with these two isolates, but increased with S. cerevisiae 2013, 2012, 2008, 2010 and 2014, with ethanol concentrations of 9.17, 8.88, 8.84, 8.44 and 8.31% (v/v), respectively. After 3 days incubation, all ten isolates survived at 40° C but only S. cerevisiae 2031 tolerated up to 15% (v/v) exogenous ethanol. S. cerevisiae 2010 and 2014 were resistant to the toxin produced by killer strain S. cerevisiae K1 (ATCC 60728). Combining the various test parameters, S. cerevisiae 2008, 2010 and 2014 would be ideal for industrial ethanol production and thus fingerprinted. Amplification of the mitochondrial small subunit ribosomal DNA (mtSSU rDNA) by polymerase chain reaction (PCR) detected polymorphisms among the S. cerevisiae strains. Cluster analysis using UPGMA-SAHN was able to group the high from the low ethanol producers but other properties such as ethanol and temperature tolerance were not distinctly separated. Repetitive sequence-based PCR analysis of the ten selected isolates using REP primers (rep-PCR) also clustered the three best S. cerevisiae isolates.

Source or Periodical Title

Philippine Journal of Science

ISSN

317683

Volume

138

Issue

1

Page

37-48

Document Type

Article

College

College of Arts and Sciences (CAS)

Language

English

Subject

Cluster analysis, DNA fingerprinting, Ethanol, Polymerase chain reaction, Saccharomyces cerevisiae

Digital Copy

YES

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