Susceptibility of the cell line Hv-AM1 from Heliothis virescens to eight selected nucleopolyhedroviruses

Issue Date

10-2008

Abstract

The cell line Hv-AM1 from Heliothis virescens was characterized for permissiveness and productivity, employing Helicoverpa armigera nucleopolyhedrovirus (HearNPV) and seven other NPVs, including those of Hyphantria cunea (HycuMNPV), Orgyia pseudotsugata (OpMNPV), Bombyx mod (BmNPV), Spodoptera exigua (SeMNPV), Spodoptera fitura (SpltMNPV), Lymantria dispar (LdMNPV) and Autographa californica (AcMNPV). Based on budded virus (BV) yields, polyhedrin production, and polyhedra formation, the results showed for the first time that the cell line Hv-AM1 was permissive for HearNPV, HycuMNPV and OpMNPV, supporting moderate levels of BVs, polyhedrin and polyhedra production. Consistent with the previous results, the cell line Hv-AM1 was also shown to be highly permissive for AcMNPV infection, yielding high levels of BVs, polyhedrin and polyhedra. In the cell line Hv-AM1 infected with BmNPV, SpltMNPV and SeMNPV, few, if any, viral progeny were produced, regardless of the severity of the observed cytopathic effects and the significant production of viral DNA, suggesting its semipermissivity nature for these NPVs. In addition, the cell line Hv-AM1 was found to readily undergo apoptosis upon infection with BmNPV, SeMNPV and HycuMNPV, while it was refractive to LdMNPV. Thus, although the cell line Hv-AM1 is not very efficient for HearNPV production, the diverse observed responses and high permissiveness against NPVs indicate that the cell line Hv-AM1 provides a novel system for analyzing the possible mechanisms underlying the permissiveness and productivity of insect cells against NPVs.

Source or Periodical Title

Journal of Insect Biotechnology and Sericology

ISSN

13468073

Volume

77

Issue

3

Page

141-150

Document Type

Article

College

College of Arts and Sciences (CAS)

Language

English

Subject

Apoptosis, Baculovirus, Heliothis virescens, Hv-AM1 cell line, NPV, Nucleopolyhedrovirus, Permissiveness, Productivity

Identifier

https://doi.org/10.11416/jibs.77.3_141

Digital Copy

YES

Link to Full Text

Share

COinS