Genetic structure and diversity of coconut (Cocos nucifera Linn.) in Southern Tagalog, Philippines as revealed by Inverse Sequence-Tagged Repeat (ISTR) analysis
Issue Date
9-2007
Abstract
This study was conducted to determine the diversity and explore the presence of discrete populations of indigenous tall coconut palms in eight locations in Santo Tomas and Tanauan, Batangas; Lukban, Mauban and Pitogo, Quezon; and Liliw, Laguna, in the Southern Tagalog region of the Philippines. Inverse sequence-tagged repeat (ISTR) analysis using four primer combinations generated 65 dominant-type markers. Shannon-Weaver diversity index, percent polymorphism and heterozygosity and genetic differentiation between population pairs were determined. High genetic diversity values were obtained within collection sites. Polymorphism within site averaged 86.15% over all populations and loci. Heterozygozity within site averaged 0.30 calculated over all loci and palms studied. Wright's fixation index (Fst) of 0.24 among populations calculated for all loci considered simultaneously was significant. Almost all pairwise values of Fst and probability of population differentiation were significant from F-statistics and Fisher's combined probability tests, respectively, indicating that populations from Banjo West, Gangahin, Novaliches and San Miguel are discrete. The Fst and differences in estimated allele frequencies were insignificant between Tinurik and Laguna Tall as well as between Santiago and Piis, resulting from movement of seednuts between localities. Shannon-Weaver diversity index among sites was low at 2.95%. A high proportion of the total diversity (97.05%) remains within populations. No geographical trends were observed among the populations based on Nei's genetic distances.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
317454
Volume
90
Issue
3
Page
196-205
Document Type
Article
Language
English
Subject
Gene flow, Genetic distances, Inverse sequence-tagged repeat analysis, Population differentiation, Shannon-Weaver diversity index
Recommended Citation
Namia, M.T.I. & Sangalang, J.B. & Altoveros, N.C. & Rodriguez, M.J.B. & Fajardo, N.N.. (2007). Genetic structure and diversity of coconut (Cocos nucifera Linn.) in Southern Tagalog, Philippines as revealed by Inverse Sequence-Tagged Repeat (ISTR) analysis. Philippine Agricultural Scientist. 90. 196-205.
Digital Copy
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