Partial purification and physicochemical characterization of glucoamylase from Aspergillus sp. BIOTECH 3104
Issue Date
6-2007
Abstract
Extracellular glucoamylase was partially purified from the culture filtrate of Aspergillus sp. BIOTECH 3104 through ultrafiltration, 90% ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography and Sephadex G-100 gel filtration. The enzyme was partially purified 21-fold at Sephadex G-100 and showed an estimated MW of 60,000 Da in SDS-PAGE. Its pH and temperature optima were pH 4.5 and 30 °C, respectively. Glucoamylase activity was significantly reduced by Co2+, Fe3+ and EDTA. Addition of 2% SDS and 0.8 M urea denatured the enzyme causing 66% and 89% loss of enzymatic activities, respectively. The use of 1% sodium benzoate and 1% potassium sorbate resulted in reduced loss of activity; with the enzyme retaining 92% and 86% of activity compared with 70% of control after 4 wk of storage at 4 °C.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
317454
Volume
90
Issue
2
Page
131-136
Document Type
Article
Language
English
Subject
Aspergillus, Chromatography, Denaturation, Glucoamylase, Saccharification
Recommended Citation
Dacuma, Mary & Espino, Teresita. (2007). Partial purification and physicochemical characterization of glucoamylase from Aspergillus sp. BIOTECH 3104. Philippine Agricultural Scientist. 90. 131-136.
Digital Copy
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