Determination of the most effective procedure for the immobilization of enzymes on amperometric biosensor for bonded glucose

Issue Date

6-2007

Abstract

Amperometric biosensors for bonded glucose were fabricated using screen printed carbon electrode bulk modified with manganese dioxide onto which glucosidase and glucose oxidase were immobilized by Nafion® film entrapment. Three methods of enzyme immobilization were performed (layering, mixing and sequencing of enzyme solutions). The analytical performance of the biosensor prepared by different enzyme immobilization methods was assessed in a flow injection mode using three sugar standards: sucrose, cellobiose and (-)-4-nitrophenyl-β-D-glucopyranoside at a potential of 480 mV (vs Ag/AgCl), pH 7.0 carrier solution (0.1 M phosphate buffer solution) and at a flow rate of 0.1 mL min-1. Peak heights of the current response were used to evaluate the results. Based on the results, the mixing of enzyme solutions was the most effective immobilization method because it produced the highest signal, followed by layering as the second most effective and sequencing of enzyme solutions as the third most effective method.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Volume

90

Issue

2

Page

137-142

Document Type

Article

Language

English

Subject

Bonded glucose, Glucose oxidase, Glucosidase, Nafion, Screen printed carbon electrode

Digital Copy

none

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