Extraction of genomic DNA from the lipid-, polysaccharide-, and polyphenol-rich coconut (Cocos nudfera L.)
Issue Date
9-2012
Abstract
One prerequisite Lo reliable molecular biology work is that the genomic DNA of a sample be of good quality. Coconut is quite difficult, to work on because of the high lipid and polysaccharide content of its endosperm and the high polyphenol content, of its leaves. This study aimed to determine which protocol to use and which part, of the coconut tree is most, appropriate to extract good-quality genomic DNA. Genomic DNA from the solid endosperm, was found to be of poor quality because of high levels of lipid and galactomannan contaminants. By using a modified protocol by Cheung et al. (1993) as modified by Rogers et al. (1996) and by Dellaporta et al. (1983) as modified by Datta et al. (1997), genomic DNA extracted from the young leaves of the first emergent frond provided enzymedigestible, good-quality DNA. The modification involved the use of a higher salt, concentration (2 M instead of 0.5 M) in the extraction buffer and the use of polyvinylpolypyrrolidone. Moreover, this modified protocol did not involve the use of organic solvents. © 2005 International Society for Plant Molecular Biology.
Source or Periodical Title
Plant Molecular Biology Reporter
ISSN
7359640
Volume
23
Document Type
Article
Language
English
Subject
Coconut, Genomic DNA, High salt, Lipid, Organic solvents, Polyphenols, Polyvinylpolypyrrolidone, Restriction enzyme digestion
Recommended Citation
Angeles, J.G.C., Laurena, A.C. & Tecson-Mendoza, E.M (2005). Extraction of genomic DNA from the lipid-, polysaccharide-, and polyphenol-rich coconut (Cocos nucifera L.). Plant Mol Biol Rep 23, 297–298.
Digital Copy
https://doi.org/10.1007/BF02772760