Utilization of a polyphasic approach in the taxonomic reassessment of antibiotic- and enzyme-producing Bacillus spp. isolated from the Philippines

Issue Date

7-2005

Abstract

The taxonomy of 58 locally isolated antibiotic- and enzyme-producing Bacillus isolates deposited at the Philippine National Collection of Microorganisms (PNCM)-BIOTECH was reassessed in this study using a polyphasic approach since they had been only partially identified prior to deposition in the culture collection. The isolates had 41.1-69% G + C, and possessed the characteristic diaminopimelic acid (DAP) and fatty acid methyl ester (FAMEs) properties of Bacillus species. Molecular analysis using specific PCR primers differentiated the isolates into two major groups, the Bacillus cereus group and the Bacillus subtilis group. To further differentiate these isolates, they were subjected to 39 phenotypic tests. Using the dichotomous key constructed for Bacillus, 45 isolates maintained their original identities, five were named at the species level, and 12 were re-identified and renamed. These results showed that the classical phenotypic tests allowed the reclassification of the isolates, while modern techniques of chemotaxonomy and the molecular approach led only to genus and cluster classification and confirmation. © Springer 2005.

Source or Periodical Title

World Journal of Microbiology and Biotechnology

ISSN

9593993

Volume

21

Page

635-644

Document Type

Article

Language

English

Subject

Bacillus, Chemotaxonomy, Molecular fingerprint, Phenotype, Taxonomy

Identifier

https://doi.org/10.1007/s11274-004-3567-4

Digital Copy

YES

Link to Full Text

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