Identification and characterization of bacteriocinogenic Lactobacillus plantarum BS25 isolated from Balao-balao, a locally fermented rice-shrimp mixture from the Philippines

Abstract

Out of 1,100 putative lactic acid bacteria (LAB) isolated from selected indigenous fermented foods in Luzon in the Philippines, one isolate, which possessed the largest zone of inhibition and the widest antimicrobial spectrum among the isolates, was selected for identification and characterization. Identification of the isolate was done using conventional morphological, biochemical and physiological methods, chemotaxonomic methods, as well as molecular methods. The polyphasic methodologies employed led to the identification of the bacteriocin-producing isolate as Lactobacillus plantarum. The bacteriocin production of the isolate was then further evaluated. Lactobacillus plantarum BS25 produced more bacteriocin in filter-sterilized modified MRS broth incubated at 30 C than in the steam-sterilized counterpart incubated under the same conditions. It was able to produce large amounts (1920 AU mL-1) of bacteriocin when the pH of the filter-sterilized culture media was adjusted to pH 5, 6, 7 and 8. Examination of changes in pH, cell count and bacteriocin activity revealed that the bacteriocin is produced as a primary metabolite. Production of large amounts of bacteriocin was accompanied by a large cell count and a decrease in pH to lower than 4.5.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

427-438

Document Type

Article

Subject

Bacteriocin, Balao-balao, Lactobacillus plantarum

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