Plant regeneration via direct shoot organogenesis from cotyledon and cotyledonary node explants of pole sitao (Vigna unguiculata {L.} Walp. var. sesquipedalis {L.} Koern.)

Abstract

A complete system for in vitro shoot induction, rooting and ex vitro establishment for pole sitao (Vigna unguiculata {L.} Walp. var. sesquipedalis {L.} Koern.) was established for the first time using the protocol for mungbean and other Asiatic Vigna spp. as the basis. Cotyledon and cotyledonary node (CN) explants from aseptic seedlings were cultured using Murashige and Skoog (MS) salts and B5 vitamins medium with 1.0 and 2.0 mg L-1 benzyladenine (BA). On the average, percentage shoot formation in cvs. UPL PS 1 and UPL PS 2 was higher using CN (67.6% and 80.4%) compared with using cotyledons (25.2% and 17.8%). However, higher responses of 52.5% to 60.0% were obtained when cotyledons from younger seedlings (1 to 3 d) were used. Shoot regeneration from CN explants of eight cultivars and advanced breeding lines revealed that the response is genotype-dependent with lines 62001, 62005 and 62012 exhibiting 97.0% to 100% shoot regeneration. Regenerated shoots excised from explants were rooted at high frequencies (80% to 100%) and survival of rooted regenerants in soil-coir dust mixture under greenhouse conditions ranged from 50% to 100%. Tissue-culture-derived regenerants from cvs. UPL PS 1 and UPL PS 2 and lines 62001, 62005 and 62012 were all morphologically normal, they flowered and produced pods filled with fertile seeds upon maturity.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

457-462

Document Type

Article

Subject

Asparagus bean, Micropropagation, Plant biotechnology, Tissue culture, Yard-long bean

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