Genetic variation of native horses (Equus caballus Linn.) in three localities of Laguna, Philippines based on protein/isozyme analysis

Abstract

The genetic variation of native horses (Equus caballus Linn.) ages 10-11 yr from three localities of Laguna, Philippines (Mabitac, Nagcarlan and Alaminos) was measured using serum proteins and isozymes. Starch-gel electrophoresis of 100 native horses revealed that except for AcPh1 with one genotype (AA), all the seven loci, namely, transferrin (Trf), with six genotypes (AA, BB, CC, AB, AC, BC), alkaline phosphatase (Alph) with three genotypes (AA,AB,BB), albumin (Alb) with three genotypes (AA,AB,BB), acid phosphatase2, Acph 2 with three genotypes (AA,AB,BB), and three loci for esterase (ESf1, Esf2, Est3) each with three genotypes (AA,AB,BB) were polymorphic. Heterozygosity (H) value ranged from 42.3%-71.5%. The proportion of polymorphic loci (P) was found to be the same for each of the populations with a value of 87.5%, and the average number of alleles (A) ranged from 1.0 to 1.39. Genetic variability was highest in Alaminos and lowest in Mabitac. The horse populations of Mabitac and Nagcarlan showed the highest degree of relatedness and, hence, shared the most common alleles as indicated by the highest genetic identity value (I) of 0.9247, while the populations of Alaminos and Mabitac had the lowest genetic similarity based on Nei's genetic distance.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

2013-09-01

Document Type

Article

Subject

Electrophoresis, Equus caballus Linn., Genetic polymorphism, Heterozygosity, Isozyme, Native horses

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