Genetic homogeneity of the banana-infecting strains of Ralstonia solanacearum (Smith) Yabuuchi et al. in the Philippines

Abstract

A population of 97 banana-infecting strains of Ralstonia solanacearum (Smith) Yabuuchi et al. from Southern Philippines was analyzed using restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR). Probes used in the RFLP analysis consisted of plasmids harboring the genes for extracellular polysaccharides (E36, O7, Q50), endoglucanase (pHE3), tryptophan biosynthesis (pT161), a random clone from the ginger strain (ZO4) library (pZ1217) and a cloned repetitive element pM114 from a Philippine bugtok strain. No polymorphism was detected in any of the strains in the RFLP analysis except with pM114 wherein seven had one extra band in different positions. PCR studies were also done using repetitive extragenic palindromic (REP), enterobacterial repetitive intergeneric consensus (ERIC) and BOX primers. Again, no polymorphism was detected except for two strains that differed in their fingerprint by just the addition of one band with REP primers. A more recent set of 80 strains was analyzed in 1998 using REP primers and only seven strains differed in their fingerprint by one band. With BOX primers, 93 out of 97 strains tested showed no polymorphism while the remaining four showed two different fingerprint patterns. The strains differing in their haplotype was not exclusive to either moko or bugtok strains. The general genetic homogeneity of bacterial wilt strains infecting banana is, therefore, established.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

394-402

Document Type

Article

Subject

Amplicon, Bacterial wilt, Bugtok, Clone, Probe, Ralstonia solanacearum, rep-PCR, Repetitive sequence, RFLP

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