Effects of extracellular Ca2+ on phagocytosis and intracellular Ca2+ concentrations in polymorphonuclear leukocytes of postpartum dairy cows
Abstract
The aim of this study was to determine the effects of extracellular Ca2+ concentration ([Ca2+]e) on phagocytosis and intracellular Ca2+ concentration ([Ca2+]i) in bovine polymorphonuclear leukocytes (PMNs). The experiments were performed by using blood samples from parturient paretic and clinically normal parturient cows and manipulating the [Ca2+]e in vitro. Phagocytosis by PMNs (with and without stimulation with phorbol myristate acetate and inhibition with cytochalasin B) and resting [Ca2+]i were significantly lower in parturient paretic cows. Repletion of Ca2+ in the extracellular media for the samples from these animals increased phagocytosis and resting [Ca2+]i. In the blood of clinically normal parturient cows, decreasing the [Ca2+]e decreased phagocytosis and resting [Ca2+]i in PMNs, but increasing the [Ca2+]e did not affect phagocytosis. These results suggest that the hypocalcemic condition of parturient paretic cows in vivo causes decreased phagocytosis and resting [Ca2+]i in PMNs, which may partly contribute to greater susceptibility to infection. © 2003 Elsevier Science Ltd. All rights reserved.
Source or Periodical Title
Research in Veterinary Science
ISSN
345288
Page
27-32
Document Type
Article
Subject
Extracellular calcium, Intracellular calcium, Phagocytosis, Polymorphonuclear leukocyte
Recommended Citation
Ducusin, R. J.T.; Uzuka, Y.; Satoh, E.; Otani, M.; Nishimura, M.; Tanabe, S.; and Sarashina, T., "Effects of extracellular Ca2+ on phagocytosis and intracellular Ca2+ concentrations in polymorphonuclear leukocytes of postpartum dairy cows" (2021). Journal Article. 3145.
https://www.ukdr.uplb.edu.ph/journal-articles/3145