Ontogeny of methionine-rich proteins in the developing cotyledon of soybean [Glycine max (L.) Merr.]
Abstract
At weekly intervals after flowering of soybean [Glycine max (L.) Merr.], accumulation of methionine-rich proteins (MRP) in the developing cotyledon was monitored through the in vitro radioactive labeling of methionine residues. Protein samples resolved through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were blotted on polyvinylidine difluoride (PVDF) membrane and subjected to an alkylation reaction specific for the thioether moiety of methionine. A radiolabeled reagent was used in the process. Putatively methionine-rich bands were observed through autoradiography. Accumulation of methionine-rich proteins (MRP) peaked at 5 wk after flowering, corresponding to the mid-maturation stage, and remained constant until maturation of the soybean pod. Based on the results of Southern blot analysis of soy genomic DNA that used polymerase chain reaction (PCR)-generated probes with primers designed from the N-terminal sequence of purified MRP, the gene encoding the MRP is unique and is expressed as 1-2 copies only.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
317454
Page
266-272
Document Type
Article
Subject
Albumin, Methionine, Ontogeny, Protein, Southern blot analysis, Soybean
Recommended Citation
Revilleza, M. Jamela R.; De Lumen, B. O.; Galvez, A. G.; Krenz, D. C.; and Mendoza, E. M.T., "Ontogeny of methionine-rich proteins in the developing cotyledon of soybean [Glycine max (L.) Merr.]" (2021). Journal Article. 3181.
https://www.ukdr.uplb.edu.ph/journal-articles/3181