Development of molecular markers for marker-assisted breeding for bacterial wilt resistance in tomato

Abstract

Since phenotypic selection of lines resistant to bacterial wilt caused by Ralstonia solanacearum has been very difficult due to the complexity of resistance expression and the considerable influence of the environment and strains, the study aimed to integrate molecular marker technologies into our breeding program. Polymerase chain reaction (PCR)-based DNA markers such as amplified fragment length polymorphism (AFLP), resistance gene analogs (RGA), and simple sequence repeats (SSR) or microsatellites were evaluated and used to construct a molecul̀ar linkage map in tomato 'Hawaii 7996' × 'WVa700' using F6 recombinant inbred lines (RIL). High-resolution detection was achieved using the non-radioactive silver staining detection method. Average marker polymorphism was 9.0% for AFLP, 10.4% for RGA, and 14.3% for SSR. A molecular linkage map, consisting of 12 linkage groups and 80 markers (72 AFLP, 7 RGA and 1 SSR), was constructed using MAPMAKER software. Map length spans a total of 378.1 cM, which is about a third of the published RFLP-based map of tomato.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

170-181

Document Type

Article

Subject

Amplified fragment length polymorphism, Bacterial wilt, DNA markers, Microsatellites, Resistance gene analogs, Simple sequence repeats, Tomato

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