Isolation, purification, and partial characterization of a lectin from the leaves of Schefflera odorata (Blanco) Merr. and Rolfe

Abstract

A lectin was extracted from the leaves of Schefflera odorata (Blanco) Merr. and Rolfe with 0.02 M phosphate buffer (pH 7.2) containing 0.15 M NaCl. The lectin was purified by ammonium sulfate precipitation and gel filtration on Sephadex G-150. The lectin was found to be both non-blood-type-specific and non-blood-group-specific because it agglutinated the erythrocytes from human blood types A, B, AB and O and those from the bloods of carabao, calf and goat. The lectin was found to be a glycoprotein containing 2.33% total sugars. The carbohydrate specificity of the lectin was not directed toward any of the sugars tested. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified lectin yielded a single protein band with an estimated molecular weight of 217 kD.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

192-196

Document Type

Article

Subject

Lectin, Schefflera odorata

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