Rapid detection of Ralstonia solanacearum (E.F. Smith) Yabuuchi in banana using a new nucleic acid-based monoclonal antibody technique

Abstract

An immunological method using monoclonal antibodies was developed against a cloned repetitive element from the 'Bugtok' banana strain of Ralstonia solanacearum (E.F. Smith) Yabuuchi. The technology was applied in detecting R. solanacearum from field-collected plant parts of banana and other species. The pathogen was detected in a large proportion of the banana samples screened, especially those collected from Mindanao, Southern Philippines. The technology was also applied in indexing 208 banana plants from Cavite and Laguna provinces near Manila, and from the orchard nursery of the Department of Horticulture, University of the Philippines Los Baños (UPLB). A positive reaction for the pathogen was noted in 44 of the 208 plants. The rapid detection of R. solanacearum makes possible the elimination of infected mother plants in tissue culture, and the interception of infected plants in the course of movement of materials in local or international trade.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

305-310

Document Type

Article

Subject

Detection, Enzyme-linked immunosorbent assay, Indexing, Monoclonal antibodies, Ralstonia solanacearum

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