Viability of in vitro matured bovine oocytes cryopreserved in a simple vitrification solution

Abstract

In vitro matured bovine oocytes were vitrified in a solution containing 7.2 M ethylene glycol as the sole cryoprotectant after equilibration in a 2 M ethylene glycol for 2, 5 or 10 min. After warming, the oocytes were subjected to in vitro fertilization using frozen-thawed semen from a Japanese Black bull. The results showed decreased viability of the oocytes exposed to ethylene glycol without vitrification as shown by lower development rates to two-four cell embryos and blastocyst stage compared with the control. Development rates of vitrified-warmed bovine oocytes fertilized in vitro that were equilibrated in 2 M ethylene glycol for 5 or 10 min and in 7.2 M ethylene glycol for 30 sec before vitrification, were significantly higher than that with 2 min of equilibration. The development rates, however, of the vitrified-warmed oocytes were significantly lower than the control oocytes. The highest blastocyst development rate of 10% of vitrified oocytes in this study is comparable with if not higher than that of other reports using different vitrification solutions or the conventional slow freezing method.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

230-237

Document Type

Article

Subject

Ethylene glycol, In vitro fertilization, In vitro matured oocytes, Viability, Vitrification

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