Isolation of banana plantlets cv. señorita freed of banana bract mosaic virus

Abstract

Banana cv Señorita, susceptible to banana bract mosaic virus, was subjected to studies on virus elimination through several possible routes including heat treatment, in vitro shoot culture, meristem culture and combinations of different lengths of heat therapy on sources of isolated meristems subsequently cultured in vitro. In BBMV-infected banana suckers, further micropropagation by shoot culture led to BBMV-infected propagules. Meristem culture and heat treatment, used as separate technologies for possible virus elimination, yielded 61.5% and 38.9 - 46.7% BBMV-free populations, respectively. Heat treatment of infected shoot cultures for 2 - 8 weeks prior to regeneration of plants from their isolated meristems yielded higher percentages of BBMV-free plants (63.2 -100%). Among the different combinations evaluated, heat treatment of micropropagated plants for even 1 week coupled with efficient serological indexing techniques was the fastest route to obtain BBMV-free plants. The implications of the results on micropropagation laboratories for banana are discussed.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

25-34

Document Type

Article

Subject

Banana, Banana bract mosaic, Heat therapy, Meristem culture, Tissue culture, Virus elimination

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