Isolation, purification and partial characterization of potato y potyvirus (PYV) in the Philippines

Abstract

Potato Y potyvirus (PYV), isolated from symptomatic potato samples collected from Benguet and Laguna, was identified through serological reactions in ELISA and through host range studies. The dilution end point of PYV was 10-2 to 10-3. The thermal inactivation point was 55 - 65 C and the longevity in vitro of PYV was 3-8 days. The molecular weight of the viral coat protein was about 34,000 daltons. The virus particles were flexuous, about 735 nm × 11 nm. Among the PYV isolates, 3 strains namely, the common strain (PYVo), veinal necrosis strain (PYVN), and stipple streak strain (PYVC) were identified. PYVo was the most prevalent strain isolated. The virus was purified through CsCI gradient centrifugation; the yield of purified PYV was about 42.60 mg/kg fresh leaf samples. An antiserum with a titer between 10-6 to 10-7 in ELISA was produced and able to detect the presence of PYV in field samples of potato, tomato, and pepper.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

97-110

Document Type

Article

Subject

Potato, Potyvirus, Purification, Serology

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