Development of the components of a cytoplasmic male sterility hybrid system in rye through anther culture

Abstract

Anther culture was applied as a method to develop the essential components of a cytoplasmic male sterility hybrid system in rye (Secale cereale L.). These components are the male sterile seed parent (A line), its isogenic maintainer counterpart (B line) and the restorer pollen parent (R line), Australian rye cultivars were crossed reciprocally to the cultivar 'Luchs' which carries the Pampa male sterile cytoplasm (cms-P). Anthers of the F1s in the cms-P cytoplasm (primary cross) and their reciprocals in the normal cytoplasm (reciprocal cross) were cultured in a modified C17 medium. Male sterile and male fertile doubled haploids were obtained from the anther culture of the F1s in the cms-P cytoplasm. Testcrosses indicated that the male sterile doubled haploids were A lines and the male fertile doubled haploids were R lines (restorers). The anther culture of genotypes in the normal cytoplasm (reciprocal cross) gave all male fertile doubled haploids. Testcrosses indicated that the male fertile doubled haploids were R lines (restorers) in the normal cytoplasm. The expected maintainer B lines were not identified because of the limited number of doubled haploids obtained from the anther culture of reciprocal crosses. Experimental single cross hybrids between male sterile and restorer male fertile doubled haploids showed high levels of heterosis. The results of this study have significant economic implications especially in the production of hybrids in several species.

Source or Periodical Title

Euphytica

ISSN

142336

Page

151-160

Document Type

Article

Subject

Anther culture, Cereal rye, Cytoplasmic male sterility, Doubled haploids, Hybrids, Secale cereale

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