Development of a rapid method for the detection of organophosphate and carbamate insecticides using enzyme inhibition technique

Abstract

An enzymatic inhibition technique sensitive for the detection of carbamate and organophosphate insecticides was developed and packaged. The sensitivity of the purified acetylcholinesterase (AChE) from the Taiwan Agricultural Research Institute (TARI) housefly head was compared with that of the crude pig liver AChE which detected as low as 0.0016μg and .014 μg of insecticide, respectively. Statistical analysis using Duncan Multiple Range Test showed that they are significantly different at 5% level. TARI's housefly head AChE was used in determining the minimum detectable limit (MDL) of the organophosphate and carbamate insecticide residues in string bean (Phaseolus sesquipedalis) samples. The enzyme inhibition technique was packaged into a rapid test kit which consists of the following: substrate reagent capsules, powdered enzyme capsules, tris buffer, acetone, ethanol, enzyme solution container-substrate solution container, extraction vials and filter paper strips. Applicability of the method was used on spiked samples extracted with acetone. A white spot indicated the presence of the insecticides while a blue spot indicated its absence.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

317454

Page

217-223

Document Type

Article

Subject

Acetylcholinesterase, Carbamates, Enzyme inhibition, Organophosphates, Rapid detection

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