Embryo rescue culture medium for improved synthesis of 4x wheat × rye hybrids
Abstract
The cross-incompatibility barrier between 4x wheat and rye has limited the genetic base for triticale breeding. Experiments designed to improve the synthesis of wheat-rye amphihaploids were conducted. The effects of 2, 4-D on crossability and 3x hybrid embryo differentiation, and the influence of one-step and two-step media on the culture of immature 3x embryos in vitro, were investigated. Application of 10 mg L-1 2, 4-D slightly improved seed set but significantly reduced the frequency of normal embryos. In contrast to the reported favourable effect of 2, 4-D on haploid embryo formation in wheat × maize crosses, the application of 2, 4-D in the present study offers no real advantage on amphihaploid embryo formation from 4x wheat × rye crosses. For small and immature wheat-rye hybrid (3x) embryos, optimum recovery in vitro was obtained via a two-step procedure consisting of a semi-solid MN medium followed by MS medium supplemented with IAA (1 mg L-1) and BAP (1 mg L-1). For bigger and well-differentiated embryos, the use of a one-step Gamborg’s B5 medium was sufficient. © 1994 CSIRO. All rights reserved.
Source or Periodical Title
Australian Journal of Botany
ISSN
671924
Page
493-500
Document Type
Article
Recommended Citation
Balatero, C. H. and Darvey, N. L., "Embryo rescue culture medium for improved synthesis of 4x wheat × rye hybrids" (2021). Journal Article. 3621.
https://www.ukdr.uplb.edu.ph/journal-articles/3621