Galactomannan degrading enzymes in maturing normal and makapuno and germinating normal coconut endosperm

Abstract

β-Mannosidase activity in the maturing normal endosperm of coconut decreased from 1.40 milliunit/mg protein at 7-8 months to 0.29 milliunit/mg protein at 11-12 months. It was 6-10 times lower at 7-8 and 8-9 months and was not detected at the more mature stages of makapuno. In contrast, the liquid endosperm from normal nuts increased in α-d-galactosidase, β-mannanase and β-mannosidase activity as development progressed. In makapuno liquid endosperm, the values for all the enzymes were consistently low during endosperm maturation. During germination, α-d-galactosidase activity in the solid endosperm increased from 0.51-0.84 unit/mg protein. β-Mannanase and β-mannosidase activity increased from 33.12 to 67.76 milliunit/mg protein and 5,13 to 43.05 milliunit/mg protein, respectively. In the haustorium, α-d-galactosidase activity remained at 0.25-0.27 unit/mg protein while β-mannanase increased from 55.34 to 138.12 milliunit/mg protein. β-Mannosidase decreased from 371.79 to 165.17 milliunit/mg protein. The implications of enzyme levels of α-d-galactosidase, β-d-mannanase and β-d-mannosidase on the mode of galactomannan degradation in the developing and germinating nut and phenotype determination of both normal and makapuno nuts are discussed. © 1989.

Source or Periodical Title

Phytochemistry

ISSN

319422

Page

2269-2273

Document Type

Article

Subject

coconut, Cocos nucifera, makapuno., α-d-galactosidase, β-mannanase, β-mannosidase, galactomannan

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