Winged bean lipoxygenase-Part 1: Isolation and purification

Abstract

Lipoxygenase was isolated and purified from dried winged bean seeds by ammonium sulphate fractionation, gel filtration, DEAE-Sephadex ion exchange chromatography and hydroxyapatite chromatography. Two major isoenzymes, FI and FII, were separated by ion exchange chromatography and were further purified by elution through a hydroxyapatite column. These resulted in a 105- and 171-fold purification and 7% and 9% recovery for FI and FII, respectively. FI and FII had similar Rf values of 0.25 on polyacrylamide gel electrophoresis. A minor band of Rf 0.01 was detected in ammonium sulphate fractions but was not further enriched and purified in succeeding steps. © 1982.

Source or Periodical Title

Food Chemistry

ISSN

3088146

Page

187-201

Document Type

Article

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