Characterization and inhibition of invertases in sugar cane juice

Abstract

(NH4)2SO4 fractionation followed by Sephadex G-200 chromatography of sugar cane juice gave an acid invertase with MW of 380 000 and 23.5% carbohydrate and a neutral invertase with MW of 66 000 and 22% carbohydrate. For acid invertase, Km is 2.8 mM and Vmax is 2.7 μmol sucrose hydrolysed/hr/mg protein. For neutral invertase, Km and Vmax are 0.32 mM and 2.8 μmol hydrolysed/hr/mg protein, respectively. Inhibition of both invertases by either lauryl sulfate or metasilicate is not competitive. © 1977.

Source or Periodical Title

Phytochemistry

ISSN

319422

Page

443-445

Document Type

Article

Subject

Gramineae, invertase inhibition, isoenzymes., Saccharum officinarum, sucrose inversion, sugar cane

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