Characterization and inhibition of invertases in sugar cane juice
Abstract
(NH4)2SO4 fractionation followed by Sephadex G-200 chromatography of sugar cane juice gave an acid invertase with MW of 380 000 and 23.5% carbohydrate and a neutral invertase with MW of 66 000 and 22% carbohydrate. For acid invertase, Km is 2.8 mM and Vmax is 2.7 μmol sucrose hydrolysed/hr/mg protein. For neutral invertase, Km and Vmax are 0.32 mM and 2.8 μmol hydrolysed/hr/mg protein, respectively. Inhibition of both invertases by either lauryl sulfate or metasilicate is not competitive. © 1977.
Source or Periodical Title
Phytochemistry
ISSN
319422
Page
443-445
Document Type
Article
Subject
Gramineae, invertase inhibition, isoenzymes., Saccharum officinarum, sucrose inversion, sugar cane
Recommended Citation
Del Rosario, Ernesto J. and Santisopasri, Vilai, "Characterization and inhibition of invertases in sugar cane juice" (2021). Journal Article. 3811.
https://www.ukdr.uplb.edu.ph/journal-articles/3811