Isoenzymes of soluble starch synthetase from Oryza sativa grains

Abstract

Two isoenzymic fractions of soluble ADP-glucose: α-1,4-glucan-4-glucosyltransferase were obtained from developing (non-waxy) rice grains by gradient elution through DEAE-cellulose. After Sephadex G-200 chromatography, fractions I and II were electrophoretically homogeneous and have MW values of 110000 and 69000, respectively. Sodium dodecyl sulfate gel electrophoresis of fraction I produced five bands with MW of 12000, 26000, 50000, 70000, and 105000 while fraction II gave two bands with MW of 12000 and 22000. Fraction II, which contains 1·7% carbohydrate, was active in the absence of added primer while fraction I, which does not contain carbohydrate, required primer. © 1976.

Source or Periodical Title

Phytochemistry

ISSN

319422

Page

71-73

Document Type

Article

Subject

ADP-glucose: α-1, 4-glucosyltransferase, Gramineae, isozymes., Oryza sativa, rice, starch synthesis

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