Large scale enterohemorrhagic E coli population genomic analysis using whole genome typing reveals recombination clusters and potential drug target

Issue Date

1-2019

Abstract

Enterohemorrhagic Escherichia coli continues to be a significant public health risk. With the onset of next generation sequencing, whole genome sequences require a new paradigm of analysis relevant for epidemiology and drug discovery. A large-scale bacterial population genomic analysis was applied to 702 isolates of serotypes associated with EHEC resulting in five pangenome clusters. Serotype incongruence with pangenome types suggests recombination clusters. Core genome analysis was performed to determine the population wide distribution of sdiA as potential drug target. Protein modelling revealed nonsynonymous variants are notably absent in the ligand binding site for quorum sensing, indicating that population wide conservation of the sdiA ligand site can be targeted for potential prophylactic purposes. Applying pathotype-wide pangenomics as a guide for determining evolution of pharmacophore sites is a potential approach in drug discovery.

Source or Periodical Title

F1000Research

ISSN

2046-1402

Volume

8

Issue

33

Page

1-11

Document Type

Article

Physical Description

illustrations, figures, tables, references

Language

English

Subject

EHEC, Escherichia coli, Pangenome, Pharmacophore

Identifier

DOI:10.12688/f1000research.17620.3

Digital Copy

yes

En – AGROVOC descriptors

EHEC; ESCHERICHIA COLI; PANGENOME; PHARMACOPHORE

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