The andrographolide content and biological activities of the ethanolic leaf extract of andrographis paniculata (Burm. f.) Ness

Abstract

© 2021 World Research Association. All rights reserved. Andrographolide is a crystalline diterpene lactone present in Andrographis paniculata (AP) and considered as the most active and important secondary metabolite component of the said plant. Few reports are available regarding the biological activities of the AP plant extract. In this study andrographolide content of ethanolic extracts of different AP plant parts was determined using UPLC. COX-2 inhibitory activity and anti-oxidant property of the extract with highest andrographolide content were examined. COX-2 inhibition assay was done using fluorometric method and antioxidant activity was determined using DPPH method. Highest content of andrographolide was observed in the ethanolic leaf extract. 150, 300, 600, 800 and 1000 µg/ml of ethanolic leaf extract of AP exhibited above 50% COX-2 inhibition and statistical analysis showed significant difference between their respective percent inhibition and the negative control. On the other hand, 100, 500, 750, 1000 and 2000 µg/ml of ethanolic leaf extract of AP exhibited 12.23, 27.08, 40.9, 46.85 and 51.67 % DPPH scavenging activity.

Source or Periodical Title

Research Journal of Chemistry and Environment

ISSN

9720626

Page

107-112

Document Type

Article

Subject

Andrographis paniculata andrographolide, Anti-inflammatory, COX-2, DPPH

This document is currently not available here.

Share

COinS