Phenotypic and molecular characterization of tungro resistant introgression lines derived from the cross Oryza sativa L. x Oryza rufipogon GRIFF

Issue Date

4-2014

Abstract

Eight out of 20 alien introgression lines (AILS) including the Philippine National Seed Industry Council recommended rice variety Matatag 9, from crosses of rice variety IR64 with five accessions of Oryza rufipogon, were found resistant to tungro infection under field conditions. Subsequent enzyme-linked immunosorbent assay (ELISA) showed that two of these visually-observed resistant AILs were resistant. (up to 30% infection to both rice tungro spherical virus (RTSV) and rice tungro bacilliform virus (RTBV), causing the rice tungro disease. Introgressions in the AILs was analyzed using 142 simple sequence repeats (SSR) markets which showed 73-80 (51-56%) polymorphism between IR64 and five accessions of O. rufipogon. Introgressions from O. rufipogon based on these markers ranged 4-16%. IR84680-74-B-B-B observed to be resistant in the field and by ELISA showed 5% (4/79 markers) introgressions from O. rufipogon only on chromosome 3. Matatag 9 showed 9% (7/78 markers) introgressions from its O. rufipogon parent which were distributed on chromosomes 1, 3, 7 and 12. The markers identified in resistant lines may potentially tag novel quantitative trait loci (QTL) for resistance to rice tungro disease (RTD) which could be of great value for further elucidation of the molecular genetic control of traits investigated. These markers can be further validated in a mapping population and can be used for fine mapping QTLs, and eventually use in MAS to accelerate breeding tungro tolerant varieties.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

39

Issue

1

Page

1-10

Document Type

Article

Physical Description

illustrations; graphs; tables

Language

English

En – AGROVOC descriptors

ORYZA RUFIPOGON; ORYZA SATIVA; RICE; GENETIC MARKERS; VARIETIES; TUNGRO DISEASE; RICE TUNGRO VIRUS; DISEASE RESISTANCE; INTROGRESSION

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