Detection of nigrospora sphaerica in the philippines and the susceptibility of three hylocereus species to reddish-brown spot disease
Issue Date
9-2020
Abstract
Diseases are among the major problems that negatively affect dragon fruit profitability worldwide. Diseases of dragon fruit in the Philippines are yet to be identified and reported. This study elucidates the causal agent of a disease infecting stems of dragon fruit grown in Los Baños, Laguna, Philippines. The fungus was isolated and identified as Nigrospora sp. based on morphological and cultural characteristics in potato dextrose agar medium. Using the DNA sequence of the internal transcribed spacer (ITS) gene region, isolate MBDF0016b was identified as Nigrospora sphaerica. The Philippines strain was closely related to the Malaysian strain, which also causes reddish-brown spot in dragon fruit (H. polyrhizus), and to other N. sphaerica isolates from other host-plant species. Nigrospora sphaerica MBDF0016b was pathogenic to H. megalanthus, H. undatus, and H. polyrhizus in detached stem and glasshouse assays. The same fungus was re-isolated from the inoculated stems and thus, establishing Koch’s postulate. This paper is the first confirmed scientific record of a dragon fruit disease in the Philippines and the first report of N. sphaerica as a dragon fruit pathogen causing reddish-brown spot disease in H. megalanthus.
Source or Periodical Title
Journal of the Professional Association for Cactus Development
ISSN
1938-663X
Volume
22
Page
49-61
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Physical Description
illustrations, tables, references
Language
English
Subject
Dragon fruit, H. megalanthus, H. polyrhizus, H. undatus, ITS gene
Recommended Citation
Taguiam, J.D., Evallo, E., Bengoa, J., Maghirang, R., & Balendres, M.A. (2020). Detection of Nigrospora sphaerica in the Philippines and the susceptibility of three Hylocereus species to reddish-brown spot disease. Journal of the Professional Association for Cactus Development, 22, 49-61. 10.56890/jpacd.v22i.321.
Identifier
10.56890/jpacd.v22i.321.
Digital Copy
yes
En – AGROVOC descriptors
DRAGON FRUIT; H. MEGALANTHUS; H. POLYRHIZUS; H. UNDATUS; ITS GENE