Genetic profile, diversity, and relationship within and among families in two generations of Eucalyptus deglupta x pellita hybrids in northern Mindanao, Philippines

Issue Date

1-2019

Abstract

Eucalyptus deglupta x pellita is among the best-performing Eucalyptus in many field trials in the country. Aside from growth performance evaluation, assessing the generational genetic potentials through DNA analysis is crucial as inbreeding is known to occur among Eucalyptus and to ensure that high genetic diversity is maintained for the next selection cycle. This study assessed the genetic diversity and relationships within and among families in two generations of Eucalyptus deglupta x pellita using the Random Amplified Polymorphic DNA (RAPD) analysis. Three independent screening using 15 primers in three parents (F1) and 74 progenies (F2) yielded 490 reproducible bands with maximum value detected in the F2. Banding patterns revealed a monomorphic genetic structure in F1 while polymorphic in F2. Polymorphism displayed high genetic variation in the F2 while EDP2F2 demonstrated superior genetic diversity within the family. The dendrograms showed high degree of relatedness in the F2 as depicted by the shrinking genetic distances from 0–15 (F1) to 0–1 (F2). The dendrogram also indicated that EDP1F1 may have pollinated the F1 as it clustered together with the F2. Genetic variants and constructed dendrograms suggested that the F1 were relatives while narrowing genetic distance was detected in the F2. This RAPD study concludes the genetic relatedness of the F2 to EDP1F1 and highest genetic potential of EDP2F2; hence, future selection could be done within this family.

ISSN

2782-8824

Volume

1

Issue

1 & 2

Page

1-12

Document Type

Article

Frequency

irregulary

Physical Description

illustrations; graphs; tables

Language

English

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