Antioxidant and physico-chemical properties and botanical origin of pollen collected by Apis cerana Frabricius from selected apiaries in Laguna and Batangas provinces (Philippines)

Issue Date

7-2019

Abstract

The physico-chemical and antioxidant properties of the ethanolic extracts of pollen collected by the Asian honey bee, Apis cerana Fabricius, from Laguna and Batangas Provinces (Luzon I., Philippines) were determined. The botanical origin of the bee pollen samples was also identified. The percent mositure ranged from 19.50 ± 0.17 to 22.44 ± 0.13; % of ash, from 2.89 ± 0.02 to 3.58 ± 0.01; % crude fat, from 0.75 ± 0.12 to 2.70 ± 0.09; % crude fiber, from 1.07 ± 0.16 to 2.76 ± 0.14; % crude protein, from 15.15 ± 0.02 to 20.49 ± 0.95; % total carbohydrates (nitrogen-free extract), from 52.67 ± 0.90 to 56.03 ± 0.09; % sugar, i.e., total sugars, reducing sugar and apparent sucrose, from 9.63 ± 1.35 to 17.30 ± 2.14; from 7.28 ± 3.09 to 14.68 ± 3.50; and from 2.24 ± 2.20 to 2.62 ± 1.54, respectively; phosphorus content, from 1839.7 ± 43.7 to 2062.9 ± 7.5 mg P/kg; and sodium content, from 14.13 ± 0.14 to 33.57 ± 0.91 mg Na/kg. In vitro determination of antioxidant properties of the bee pollen samples includes 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging Capacity with EC50 values of 7.86 ± 0.11 mg/mL to 8.02 ± 0.08, Hydrogen Peroxide Scavenging Capacity with EC50 of 30.86 ± 0.07 to 31.87 ± 0.09 µg/mL and Ferric Ion Reducing Antioxidant Power (FRAP) values of 2.69 ± 0.37 to 3.01 ± 0.49 mmol Fe2+/g extract. All bee pollen samples were multifloral with 19 different pollen types. Variations on the values obtained were attributed to the variation of geographical location and floral sources of the pollen in the samples.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

33

Issue

1

Page

31-47

Document Type

Article

College

College of Arts and Sciences (CAS)

Frequency

semi-annually

Physical Description

illustrations; graphs; references

Language

English

Subject

Apis cerena; Pollen; Honey

En – AGROVOC descriptors

APIS CERANA; APIDAE; HONEY BEES; APIARIES; PROXIMATE ANALYSIS; SITE; CHEMICOPHYSICAL PROPERTIES; POLLEN; ANTIOXIDANTS; PROVENANCE; BIOGEOGRAPHY; PHILIPPINES

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