Rice dwarfs virus (RDV) detection in the Nephotettix nigropictus (Stål) and rice plant, and field reaction of rice cultivars against RDV in the Philippines

Issue Date

1-2019

Abstract

The rice dwarf disease generally occurs in temperate rice growing countries. Its occurrence in the Philippines was reported in 1994 based on transmission studies, electron microscopy of the virus particles, and serological assay of infected plants. A simple nucleic acid amplification technique called the loop-mediated isothermal amplification (LAMP) was applied to detect Rice Dwarf virus (RDV) in field collected insect vector, Nephotettix nigropictus (Stål), individuals and rice plants. Samples were crudely lysated with alkaline lysis method. LAMP successfully detected RDV in both insect vector and plant hosts at 60 ˚C in 60 min. Visual scores of rice dwarf diseased plants in the National Cooperative Trial (NCT) 2014 dry season test showed that rice lines developed for the upland ecosystem obtained the highest infection rate (13.89%); nearly as high as the TN1 check plants (15.21%) while the Multi-Adaptation Trial (MAT) lines obtained the lowest (059%). In the 2015 wet season NCT tiral, the Traditional rice group recorded the highest percentage of infection (4.81%) followed by those in the direct wet seeded rice (DWSR) category (3.47%) while rice entries in MAT and Transplanted categories obtained the two lowest infection of 1.20% and 1.25%, respectively. The TN1 check variety recorded 2.54 % infection. A 0.4% rice dwarf disease incidence was recorded in a field survey in the rice production area of the PhilRice Midsayap Branch station during the 2015 DS cropping. The average number of N. Nigropictus in the NCT and rice production fields ranged from 0-4.33 for 10 insect net sweeps.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

33

Issue

1

Page

61-73

Document Type

Article

Frequency

semi-annually

Physical Description

Illustrations; tables; references

Language

English

Subject

Rice disease and pest resistance

En – AGROVOC descriptors

ORYZA SATIVA; RICE; NEPHOTETTIX NIGROPICTUS; RICE DWARF VIRUS; LEAF EATING INSECTS; PEST INSECTS; PEST OF PLANTS; PLANT DISEASES; PLANT VIRUSES; INFESTATION; NUCLEIC ACIDS; NUCLEIC PROBES

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