Issue Date
10-2017
Abstract
The primary endosymbionts associated with coconut scale insects (CSI), Aspidiotus rigidus and A. destructor, were detected using the 16S rRNA. Sequences of this gene were sourced from A. destructor and A. rigidus populations from different geographical locations namely, Orani, Bataan; Cagbalate 1 and Cagbalete 2, Mauban, Quezon; Sampaloc, Quezon; and Tranca, Bay, Laguna. Results revealed variation between the primary endosymbiont associated with the Aspidiotus species, but not among the primary endosymbionts associated within each CSI species. GenBank mining using BLAST revealed 98% to 99% similarity of the endosymbionts of A. destructor and A. rigidus, respectively, with Candidatus Uzinura diaspidicola (GenBank Acc. No. DQ868800.1), - the identified primary bacterial endosymbiont 16S rRNA gene comparison, inferred that the U. diaspidicola of the two CSI species were entirely different and thus, could serve as a target in designing sound management strategies against these economically important pests. With the various vital roles of endosymbionts in their host ecology and evolution, more in-depth studies on U. diaspidicola could definitely help understand the mechanism of relative higher invasiveness of A. rigidus over A. destructor in coconut. Moreover, our results suggest that, with the difficulties encountered through conventional techniques of species identification, molecular-based approach such as utilization of 16S rRNA gene of an endosymbiont could be an efficient additional tool for a more accurate and faster identification of scale insect species.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
31
Issue
2
Page
117-127
Document Type
Article
Frequency
semi-annually
Physical Description
illustrations
Language
English
Recommended Citation
Timple, Jillian E.; Latina, Romnick A.; and Caoili, Barbara L., "Molecular marker-based detection of primary bacterial endosymbiont associated with the coconut scale insects, aspidiotus destructor Signoret and aspidiotus rigidus reyne (Hemiptera: Diaspididae)" (2017). Journal Article. 4036.
https://www.ukdr.uplb.edu.ph/journal-articles/4036
En – AGROVOC descriptors
ASPIDIOTUS, COCCODEA, DIASPIDIDAE, GENETIC MARKERS, INSECT CONTROL, NUCLEOTIDE SEQUENCE AND RNA, NUCLEOTIDE SEQUENCE, IDENTIFICATION