Research Note: Modified methods for histological study of galls induced by Pauropsylla deflexa Uichanco (Hemiptera: Triozidae) on leaves of Ficus ulmifolia Lam. (Moraceae)

Issue Date

10-2017

Abstract

Leaf galls on Ficus ulmifolia Lam. (Moraceae) caused by the jumping plant lice, Pauropsylla deflexa Uichanco (Hemiptera: Sternorrhyncha: Psylloidea: Triozidae), are commonly observed in various locations in Mt. Makiling, Laguna, Philippines. Normal and galled leaves were collected for anatomical-histological studies. Samples were fixed, embedded in paraffin, sectioned, and stained with safranin and fast green. During processing, modifications of standard protocols were deemed necessary and are herewith enumerated. Measurements were done on the different tissues of the galls to compare with those of a normal leaf, and demonstrate the suitability of the modified methods for histological study of insect-induced leaf galls. The sections clearly showed that leaf galls arise from changes in the morphogenesis of the cells and tissues of plant organs. Thick layers of parenchyma were observed in cross sections of leaf galls which are modified palisade mesophyll cells in normal leaves. Other modifications on the leaf gall are apparent adaptations to protect the insect inside its chamber.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

129-137

Document Type

Article

Frequency

semi-annually

Physical Description

illustrations ; tables

Language

English

En – AGROVOC descriptors

MORACEAE; TRIOZIDAE; PSYLLOIDEA; CECIDOMYIIDAE; THYSANOPTERA; LEAF EATING INSECTS; PEST INSECTS; PESTS; PESTS OF PLANTS; PLANT GALLS; PLANT DISEASES; GALL CAUSING INSECTS; PLANT TISSUES; PLANT ANATOMY; LEAVES; MESOPHYLL; MORPHOGENESIS; PARENCHYMA

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