Rapid multiplex PCR-based protocol for aspidiotus rigidus reyne detection
Issue Date
10-2017
Abstract
Different studies focusing on Aspidiotus species infesting coconuts have been rapidly increasing in the country as a result of the threatening outbreak of these armored scale insects just a few years back. Intensive morpho-taxonomic study coupled with molecular analyses revealed identity of the causal diaspidid pest as A. rigidus and not the initially identified A. destructor. The earlier non-recognition of A. rigidus as distinct from A. destructor calls for a more accurate and rapid diagnostic protocol especially for non-diaspidid experts. Using the aligned partial nucleotide sequences coding for the 28S ribosomal DNA of Aspidiotus spp., an A. rigidus-specific oligonucleotide marker based on the deletion site common to all A. rigidus samples was developed and optimized. The multiplex-based PCR protocol which was evaluated using laboratory-reared and field-collected samples provides a fast and economical detection of A. rigidus.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
31
Issue
2
Page
139
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Frequency
semi-annually
Language
English
Recommended Citation
Latina, Romnick A.; Lantican, Darlon V.; Guerrero, Michelle S.; and Caoili, Barbara L., "Rapid multiplex PCR-based protocol for aspidiotus rigidus reyne detection" (2017). Journal Article. 4039.
https://www.ukdr.uplb.edu.ph/journal-articles/4039
En – AGROVOC descriptors
COCONUTS; ASPIDIOTUS; COCCOIDEA; DIASPIDIDAE; PEST OF PLANTS; PEST, INSECTA; PEST INSECTS; NUCLEOTIDE SEQUENCE; PCR