Rapid multiplex PCR-based protocol for aspidiotus rigidus reyne detection

Issue Date

10-2017

Abstract

Different studies focusing on Aspidiotus species infesting coconuts have been rapidly increasing in the country as a result of the threatening outbreak of these armored scale insects just a few years back. Intensive morpho-taxonomic study coupled with molecular analyses revealed identity of the causal diaspidid pest as A. rigidus and not the initially identified A. destructor. The earlier non-recognition of A. rigidus as distinct from A. destructor calls for a more accurate and rapid diagnostic protocol especially for non-diaspidid experts. Using the aligned partial nucleotide sequences coding for the 28S ribosomal DNA of Aspidiotus spp., an A. rigidus-specific oligonucleotide marker based on the deletion site common to all A. rigidus samples was developed and optimized. The multiplex-based PCR protocol which was evaluated using laboratory-reared and field-collected samples provides a fast and economical detection of A. rigidus.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

139

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

COCONUTS; ASPIDIOTUS; COCCOIDEA; DIASPIDIDAE; PEST OF PLANTS; PEST, INSECTA; PEST INSECTS; NUCLEOTIDE SEQUENCE; PCR

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