Survey of secondary endosymbionts of bemisia tabaci biotypes present in the Philippines
Issue Date
10-2017
Abstract
Bemisia tabaci (Gennadius), just like many arthropods, harbors bacterial endosymbionts. These symbionts, particularly the secondary endosymbionts (S-endosymbionts) have been widely investigated because of their significant role in parasitoid resistance, virus transmission, insecticide susceptibility, and insect ecology and fitness of their whitefly hosts. Here, we did preliminary survey of the S-endosymbionts present in the different B. tabaci biotypes in the Philippines previously identified (MEAM 1, ASIA 1, AUS (Australian), ASIA II 6 and ASIA II 10). Amplification of the 16S and 23S ribosomal DNA using genus-specific primers revealed the presence of four S-endosymbionts, namely: Hamiltonella, Wolbachia, Rickettsia, and Arsenophonus. Wolbachia was abundant in all of the samples, regardless of biotype. Out of the 186 B. tabaci samples, 46.77% had single detection, 32.26% two, 8.6 % three, and 12.37% had no detection of any of the four endosymbionts. Hamiltonella was restricted to the destructive MEAM 1 biotype, where it was present in 85% of the samples. Meanwhile, Arsenophonus appears to be present solely in Asia 1 biotype. While amplicons were generated from some samples using the genus-specific primers for cardinium, Ch-f and Ch-r, nucleotide sequences surprisingly revealed the presence of a primary endosymbiont, Ca. Portiera aleyrodidarum, instead.
Source or Periodical Title
The Philippine Entomologist
ISSN
0048-3753
Volume
31
Issue
2
Page
143-144
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Frequency
semi-annually
Language
English
Recommended Citation
Sandoval, Regina Faye C.; Ogot, Aaron V.; Sapin, Gelyn D.; Latina, Romnick A.; and Caoili, Barbara L., "Survey of secondary endosymbionts of bemisia tabaci biotypes present in the Philippines" (2017). Journal Article. 4046.
https://www.ukdr.uplb.edu.ph/journal-articles/4046
En – AGROVOC descriptors
BEMISIA TABACI; INSECTA; PESTS; PEST INSECTS; PEST OF PLANTS; NUCLEOTIDE SEQUENCE; BIOLOGICAL CONTROL AGENTS; BIOTYPES; SYMBIONTS; PARASITOIDS