Survey of secondary endosymbionts of bemisia tabaci biotypes present in the Philippines

Issue Date

10-2017

Abstract

Bemisia tabaci (Gennadius), just like many arthropods, harbors bacterial endosymbionts. These symbionts, particularly the secondary endosymbionts (S-endosymbionts) have been widely investigated because of their significant role in parasitoid resistance, virus transmission, insecticide susceptibility, and insect ecology and fitness of their whitefly hosts. Here, we did preliminary survey of the S-endosymbionts present in the different B. tabaci biotypes in the Philippines previously identified (MEAM 1, ASIA 1, AUS (Australian), ASIA II 6 and ASIA II 10). Amplification of the 16S and 23S ribosomal DNA using genus-specific primers revealed the presence of four S-endosymbionts, namely: Hamiltonella, Wolbachia, Rickettsia, and Arsenophonus. Wolbachia was abundant in all of the samples, regardless of biotype. Out of the 186 B. tabaci samples, 46.77% had single detection, 32.26% two, 8.6 % three, and 12.37% had no detection of any of the four endosymbionts. Hamiltonella was restricted to the destructive MEAM 1 biotype, where it was present in 85% of the samples. Meanwhile, Arsenophonus appears to be present solely in Asia 1 biotype. While amplicons were generated from some samples using the genus-specific primers for cardinium, Ch-f and Ch-r, nucleotide sequences surprisingly revealed the presence of a primary endosymbiont, Ca. Portiera aleyrodidarum, instead.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

143-144

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

BEMISIA TABACI; INSECTA; PESTS; PEST INSECTS; PEST OF PLANTS; NUCLEOTIDE SEQUENCE; BIOLOGICAL CONTROL AGENTS; BIOTYPES; SYMBIONTS; PARASITOIDS

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