Molecular identification and preliminary genetic diversity analysis of tobacco stem borer, scrobipalpa aptatella (Walker) (Lepidoptera: Gelechiidae) from Misamis Oriental and Bukidnon using Cytochrome Oxidase Subunit I (COI) gene

Issue Date

10-2017

Abstract

Scrobipalpa aptatella (Walker) (Lepidoptera: Gelechiidae), also known as tobacco stem borer (TSB), is a reported pest of tobacco. The damage it inflicts are mainly tunnels created by larval feeding on the stem. As the infestation progresses, other symptoms appear like stem galling and wilting. With serious infestation, a plantation can lose 30-70% of field output. Proper identification of the pest is crucial in establishing effective control measures to prevent serious infestation of the TSB. DNA barcoding is an efficient way to establish the identity of a specific organism. However, there are no studies in the Philippines using this technique to identify TSB. Cytochrome c oxidase I (COI) was used as the molecular marker to identify the collected TSB from Claveria, Misamis Oriental and Bukidnon. COI gene sequences from individuals were obtained and compared with those in GenBank which revealed a 99% BLAST hit. All TSB samples point to S. aptatella (GenBank Acc. No. KF388766.1). Multiple sequences alignment of the COI gene revealed single nucleotide polymorphisms within and between the collected populations. Haplotype and nucleotide diversity of the populations had values of 0.2584 and 0.00074, respectively. Five haplotypes were identified. A cladogram showed the haplotypes through the groupings. The Mindanao population, in general, is slowly expanding, based on the negative results of Tajima’s D test of neutrality. Fu’s Fs test of neutrality showed an excess of rare haplotypes in the population. The recent population expansion suggests that there is already a need for control measures to prevent further expansion.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

144

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

GELECHIIDAE; SCROBIPALPA; INSECTA; PESTS; PEST INSECTS; PEST OF PLANTS; STEM EATING INSECTS; DNA; CYTOCHROME C OXIDASE; GENETIC MARKERS; SINGLE NUCLEOTIDE POLYMORPHISM; INSECT CONTROL; INFESTATION

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