Advanced lines of traditional corn toward breeding for resistance to the Asian corn borer, ostrinia furnacalis (Guenee) (Lepidoptera: Crambidae)

Issue Date

10-2017

Abstract

The Asian corn borer (ACB), Ostrinia furnacalis (Guenee), is still the most destructive insect pest of open-pollinated varieties (OPV’s) of maize in the Philippines. This paper reports the results of initial renewed efforts to search for native corn germplasm with inherent resistance to ACB. Two hundred eighty-seven S1 advanced traditional maize lines were generated in 2016. These were evaluated through no-choice leaf disc and stalk-feeding bioefficacy tests, using 2nd instar laboratory-reared ACB larvae. Test materials were taken from corn plants at vegetative (30-35 days after planting, DAP) and reproductive (40-60 DAP) stages, respectively. The S1 families, CGUARDCnN32_C0-14, CGUARDN31_C0-04 and CGUARDCnN40_C0-02 exhibited significant levels of leaf feeding and stalk feeding resistance in both assays as revealed in mean percent survival (35.33-49.33%) and the mean tunnel length (4.33-8.33 mm). Strip test for Bt Cry1Ab/Ac protein of these lines using leaf collected randomly from the field at 30-35 DAP had negative results implying inherent ACB resistance. Feeding behavior of ACB larvae when grown on promising S1 lines were noted. DIMBOA (2, 4-dihydroxy-7-methoxy-(2H)-1, 4-benzoxazin-3 (4H)-one) levels were compared in vitro for the promising lines to elucidate the mechanism of resistance of these varieties. These promising advanced lines of native corn can be utilized for maize population improvement and eventually for breeding for resistance to the ACB, especially in the tropics.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

158

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

MAIZE; TRANSGENIC PLANTS; OSTRINIA FURNACALIS; LARVAE; INSECTA; PESTS; PEST OF PLANTS; PEST INSECTS; PEST; RESISTANCE TO; GERMPLASM; OPEN POLLINATION

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