Comparison of survival rates of neonates and second instar larvae of Ostrinia furnacalis (Guenee) for larval movement studies

Issue Date

10-2017

Abstract

The behavior of neonates and early instar larvae of the Asian corn borer (ACB), Ostrinia furnacalis (Guenee), in the field is little understood. Studies along this line are needed to understand the refuge recommendation of introduced Bt corn products for insect resistance management, and in designing an appropriate refuge system that will delay the development of resistance to ACB. This study aims to generate basic information on the larval survival of ACB by introducing neonates and second instar larvae on 40-day old corn plants. This will be useful in studying larval movement of ACB in the field. ACB were collected from four locations (Katangawan, Arenas, Revas, and Conel Road) in General Santos City, Philippines and infested on the test plants, caged to prevent natural infestation of feral or wild ACB and natural enemies. Test plants from different treatments with three replications were dissected at 4, 8, 12, and 16 days after infestation (DAI). The experiment was conducted under normal field conditions. Second instar larvae at 4, 8, and 16 DAI had the highest mean larval survival compared to neonates. However, more neonates survived at 12 DAI and then both had almost the same number of larvae at 16 DAI. Although 2nd instar larvae had the highest larval survival from the start, surviving larvae were the same during the last sampling period. Hence, both neonate and second instar larvae can be used for larval movement studies. The use of blackhead-stage egg masses is also being tested.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

31

Issue

2

Page

160

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

MAIZE; INSECTA; OSTRINIA FURNACALIS; BACILLUS THURINGIENSIS; LARVAE; LARVAL STAGE; ANIMAL DEVELOPMENT STAGES; INSTARS, PESTS, PEST OF PLANTS, PEST INSECTS, PEST, RESISTANCE TO,

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