Transfer of soybean glycinin gene into rice via agrobacterium tumefaciens-mediated transformation
Issue Date
8-2005
Abstract
Soybean glycinin gene was transrerred into rice via Agrobacterium tumefaciens-mediated transformation. A tumefaciens is a pathogenic soil bacterium which is now commonly used to introduce donor DNA into plant cells to obtain transgenic plants. Embryogenic calli introduce from scutella of mature seeds served as excellent materials for transformation. Calli were infected with an A, tumefaciens strain EHA105 carrying a binary vector containing glycinin gene fused to an endosperm-specific promoter of the rice storage glutelin gene and hygromycin resistance gene (HygR). Hygrmycin (50 mg/L was used as a selectable agent. Genetic transformation was successful for the Japonica variety Kitaake, but not for the Indica varieties PSB Rc14 and PSB Rc34. Transformation effeciency was 14%. The transformation system was simple, reliable and reproducible. All transgenic plants were fertile and morphologically normal. Integration of foreign genes into the genome of transgenic plants was confirmed by polymeruse chain reaction (PCR) and northern blot analysis.
Source or Periodical Title
Philippine Journal of Crop Science
ISSN
0115-463x
Volume
30
Issue
2
Page
39-45
Document Type
Article
Frequency
tri-quarterly
Physical Description
chart. graph, table
Language
English
Recommended Citation
Lapitan, Victoria C.; Washida, Haruhiko; Tada, Yoshifumi; and Takaiwa, Fumio, "Transfer of soybean glycinin gene into rice via agrobacterium tumefaciens-mediated transformation" (2005). Journal Article. 4225.
https://www.ukdr.uplb.edu.ph/journal-articles/4225