Transfer of soybean glycinin gene into rice via agrobacterium tumefaciens-mediated transformation

Issue Date

8-2005

Abstract

Soybean glycinin gene was transrerred into rice via Agrobacterium tumefaciens-mediated transformation. A tumefaciens is a pathogenic soil bacterium which is now commonly used to introduce donor DNA into plant cells to obtain transgenic plants. Embryogenic calli introduce from scutella of mature seeds served as excellent materials for transformation. Calli were infected with an A, tumefaciens strain EHA105 carrying a binary vector containing glycinin gene fused to an endosperm-specific promoter of the rice storage glutelin gene and hygromycin resistance gene (HygR). Hygrmycin (50 mg/L was used as a selectable agent. Genetic transformation was successful for the Japonica variety Kitaake, but not for the Indica varieties PSB Rc14 and PSB Rc34. Transformation effeciency was 14%. The transformation system was simple, reliable and reproducible. All transgenic plants were fertile and morphologically normal. Integration of foreign genes into the genome of transgenic plants was confirmed by polymeruse chain reaction (PCR) and northern blot analysis.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

30

Issue

2

Page

39-45

Document Type

Article

Frequency

tri-quarterly

Physical Description

chart. graph, table

Language

English

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