PR4662A - a newly developed female parent through CMS conversion for hybrid rice development

Issue Date

7-2018

Abstract

Commercial exploitation of heterosis for hybrid rice technology would not be possible without sterility mechanism. In the Philippines, three-line system (CMS-based hybrids) is the most widely used by both public breeding institution and private companies because of its stability to varying environmental condition at the stage of F1 seed production. Male sterility in this study, the development of PR4662A, a newly-bred CMS line studied in detail for consideration in the hybrid rice breeding pool. The study was conducted at the experimental area of the Plant Breeding and Biotechnology Division of the Philippine Rice Research Institute. CMS conversion was started by selecting 35 elite maintainer (B) lines with trait of interest along with the four identified female parents (A). Hill to hill crossing was conducted to obtain the F1 seeds and series of backcrosses were followed up to six backcross generations (BC1F1 - BC6F1). Selection criteria for every backcross generation was based on the target trait from the recurrent parent, complete sterility, stability (microscope and phenotypic evaluation) and uniformity following the schematic conventional backcrossing, F! has 50% of its genome from male and 50% of unrelated genome from the female parent. BC1F1 generation has 75% recovery genome from male or recurrent parent (RP), BC2F1 has 87.5% from RP, 93.75% recovery genome for both BC3F1 and BC4F1 while 98.44% on BC5F1. Complete recovery of the recurrent parent is achieved of BC6F1. By thorough evaluation and initial testcrosses results, PR46622A can be nominated to be one of the upcoming female parents to develop superior hybrid.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463X

Volume

43

Issue

Supplement no. 1

Page

100

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Subject

Oryza sativa;Hybrid rice;

En – AGROVOC descriptors

ORYZA SATIVA; HYBRIDS; HETEROSIS; CYTOPLASMIC MALE STERILITY; SELECTION; BACKCROSSING

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