Cytotoxicity of Peperomia pellucid (L.) HBK extracts on cancer cell lines and their effects on cfos and cjun genes
Issue Date
3-2019
Abstract
The cytotoxicity of methanolic and ethanolic extracts from the plant locally known as 'Pansit-pansitan' [Peperomia pellucid (L.) HBK] on human cancer and normal cell lines was determined. Extracts were obtained by macerating fresh aerial of the plant with methanol and ethanol, followed by rotary evaporation. Extracts were dissolved in dimethylsulfoxide (DMSO) and filter-sterilized. Cytotoxicity was tested on human colorectal adenocarcinoma cells (HT-29), human monocytic leukemia cells (THP-1), and normal human fibroblast and liver cells (HDFn and THELE-3, respectively) using PrestoBlue R resazurin assay. Zeocin was used as positive control. Absolute quantification of transcript levels for the early apoptotic cfos and cjun marker genes was conducted using quantitative real time polymerase chain reaction (qRT-PCR). Cytotoxicity tests showed that based on the guidelines of the US National Cancer Institute, both extracts were highly cytotoxic to the cancer cell lines, as evidenced by their half maximal cytotoxic concentration (IC 50) values below 20 mug/mL. These ranged from 7.374 to 12.112 mug/mL. On the other hand the extracts were found to be nontoxic to the two normal cell lines HDFn and THLE-3, with (IC50) of 55.629 mug/mL, respectively, for the methanolic extract; and 52.188 mug/mL, and 63.483 mug/mL, respectively, for the ethanolic extract. Expression of the early apoptotic genes cjun and cfos was found to be upregulated in both HT-29 and THP-1 treated with (IC50) of the plant extracts. The results showed that the extracts have promising anti cancer therapeutic potential, as suggested by the upregulation of the expression of the early apoptotic genes cjun and cfos in cancer cells, without being cytotoxic to normal cells.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
0031-7454
Volume
102
Issue
1
Page
24-32
Document Type
Article
College
College of Agriculture and Food Science (CAFS)
Physical Description
graphs; table
Language
English
Subject
Medicinal plants; Plant extracts -- Therapeutic use;Ethanol
Recommended Citation
Buhian, Sarah Patricia C.; Oyong, Glenn G.; and Cabrera, Esperanza C., "Cytotoxicity of Peperomia pellucid (L.) HBK extracts on cancer cell lines and their effects on cfos and cjun genes" (2019). Journal Article. 4351.
https://www.ukdr.uplb.edu.ph/journal-articles/4351
En – AGROVOC descriptors
PEPEROMIA; SPECIES; PLANT EXTRACTS; DRUG PLANTS; NEOPLASMS; TOXICITY; METHANOL; ETHANOL; APOPTOSIS