Total phenolics, total flavonoids, antioxidant activity and antibacterial property of propolis produced by the stingless bee, Tetragonula biroi (Friese) from Laguna and Quezon, Philippines

Issue Date

4-2016

Abstract

The total phenolics (TP) and total flavonoids (TF) of the ethanolic extracts of Tetragonula biroi (Friese) propolis obtained from selected municipalities in Laguna and Quezon, Philippines were determined using Folin-Ciocalteau and aluminum chloride colorimetric methods, respectively. The antioxidant activities were determined using ferric ion reducing antioxidant power (FRAP), hydrogen peroxide scavenging activity (HPSA) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. Values obtained for Laguna propolis in each assay were 255.22 ± 14.75 mg GAE/g, 1.91 ± 0.42 mg QE/g, 5.21 ± 3.08 μmol Fe 2+ /g, 12.73 ± 1.45 (IC 50 ) and 15.49 ± 1.13 (IC 50 ), respectively. Those for Quezon propolis were 308.04 ± 119.55 mg GAE/g, 5.30 ± 1.43 mg QE/g, 24.22 ± 16.88 μmol Fe 2+ /g, 11.39 ± 2.33 (IC 50 ) and 14.94 ± 3.49 (IC 50 ), respectively. Statistical analyses showed that the samples only differed significantly in TF and FRAP values. This explains the similarity in the in vitro partial inhibitory activity and reactivity of the samples against gram-negative Escherichia coli and gram-positive Staphylococcus aureus

ISSN

0048-3753

Volume

30

Issue

1

Page

63-74

Document Type

Article

Physical Description

illustrations ; tables

Language

English

En – AGROVOC descriptors

MELIPONA; FLAVONOIDS; POLYPHENOLS; PROPOLIS; ANTIOXIDANTS; ANTIMICROBIAL PROPERTIES; PHILIPPINES

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