Cytological and cytochrome C oxidase II (COII) gene characterization of the bean podborer, Maruca vitrata (Fabricius) (Lepidoptera: Crambidae)

Issue Date

4-2016

Abstract

The bean podborer, Maruca vitrata (Fabricius) was characterized through its karyotype and mitochondrial cytochrome c oxidase II (COII) gene. The chromosomes are irregular in shape, with number ranging from 23 to 66, relative lengths of 0.010 to 0.073 and with no definite centromeric sites. The partial nucleotide sequence of the COII gene is 662 base pairs that encodes 199 amino acids. This was submitted to GenBank as Maruca testulalis (Gever), synonym of M. vitrata, and was assigned to Accession No. AY899212.1. The putative initiation codon was ATG at position 64 to 66 and encodes the amino methionine. The hene has high A-T content of 81% with A-T bias in codon usage that preferred the third position. The alignment of the COII gene with those of other members of family Crambidae that are available in GenBank, revealed that it is most related to Heliothis virescens.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

30

Issue

1

Page

75-83

Document Type

Article

Frequency

semi-annually

Physical Description

illustrations ; tables

Language

English

En – AGROVOC descriptors

MARUCA VITRATA; PESTS OF PLANTS; INSECTA; CYTOCHROME C OXISADE; KARYOTYPES; HISTOCYTOLOGICAL ANALYSIS

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