Enzyme mixtures for the extraction of oil from the seeds of vutalao (Calophyllum ino phyllum

Issue Date

12-2007

Abstract

As an alternative method, enzymatic extraction was applied to recover oil from the seeds of Alexandrian laurel or vutalao (Calophyllum inophyllum). Three crude enzymes (xylanase, a-amylase and cellulase) were used singly and in a combination for the extraction process. The enzyme mixture for maximum oil recovery was determined by Response Surface Methodology (RSM) employing simplex lattice design. Results showed that the means of oil recovery using the 3 different enzymes that had been applied singly were significantly different. Mixture optimization showed that the maximum recovery was achieved using 1:1 binary mix of xylanase and cellulase or using 1:1 binary mix of xylanase and a-amylase. Both of these mixes have a desirability index vary close to 1, with an oil recovery of 97%.

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

32

Issue

3

Page

17-30

Document Type

Article

Frequency

tri-quarterly

Physical Description

diagram, charts, tables

Language

English

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