Molecular identification of potential entomophathogenic fungi isolated from coconut scale insects (Aspidiotus spp.)

Issue Date

10-2016

Abstract

A total of 244 entomopathogenic fungal (EPF) isolates were purified from coconut scale insects (CSI) (Aspidiotus spp.) collected from 22 localities in 10 provinces (Aurora, Basilan, Bataan, Batangas, Cagayan, Cavite, Isabela, Laguna, Quezon and Zamboanga) [Philippines] from 2014 to 2016. Materials for DNA extraction were obtained from a single spore colony. The ITS-5.8s nucleotide regions were amplified but only 158 EPF samples were successfully sequenced. These nucleotide sequences were then compared with the sequences in the GenBank database using Basic Local Alignment Search Tool (BLAST) of National Center for Botechnology Information (NCBI). Some of the fungal genera identified were: 1)entomopathogenic strains, namely, Aschersonia, Fusarium and Purpureocillium; 2)the endophytes, Clanostachys and Penicillium; 3) a plant disease antagonist such as Lecanicillium, The potential entomopathogenicity of these isolates are currently being evaluated using Koch's postulates tests and bioassays.

Source or Periodical Title

The Philippine Entomologist

ISSN

0048-3753

Volume

30

Issue

2

Page

185

Document Type

Article

Frequency

semi-annually

Language

English

En – AGROVOC descriptors

COCCOIDEA; ASPIDIOTUS; ENDOPHYTES; PESTS OF PLANTS; PLANT DISEASE; ENTOMOGENOUS FUNGI

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