Plantlet regeneration from cell suspension cultures of banana cv. saba via somatic embryogenesis

Issue Date

4-2009

Abstract

Higher number of meristematic buds (scalps) proliferated at the basal region of sterile shoot tip cultures in modified Murashige and Skoog's (1962) basal medium supplement with 1 μM indoleacetic acid (IAA) and μM benzylaminopurine (BA) in less than 3 weeks of incubation than those in media with lower BA concentrations. On the other hand, higher number of scalps was obtained in 1 μM thidiazuron (TDZ) than in BA (22.2 μM) supplemented culture medium. Yellowish and glossy meristematic globules were formed from enlarged scalps in modified liquid medium consisting of ½ Murashige and Skoog (MS) macro and iron-ethylenediaminetetra-acetic acid (Fe-EDTA) with 5 μM 2,4-dichlorophenoxyacetic acid (2,3-D) and 1 μM zeatin. Browning of scalps was effectively reduced by refreshing the culture medium every 2 days for the first 2 weeks of culture. Embryogenic cell suspension cultures released from meristematic globules were induced to undergo somatic embryogenesis in liquid MS medium supplemented with 9.1 μM zeatin. Primary embryos that were transferred onto semi-solid MS + 9.1 μM zeatin formed secondary embryos. Increased rate of shoot multiplication were observed on germinated secondary embryos which were passed through four subcultures onto MS + 1 μM + 22.2 μM BA and further subcultured in MS + 15% coconut water + 22.2 μM BA (MC + 22.2 μM BA).

Source or Periodical Title

Philippine Journal of Crop Science

ISSN

0115-463x

Volume

34

Issue

1

Page

1-12

Document Type

Article

Frequency

tri-quarterly

Physical Description

tables, pictures

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